PCR amplification of specific DNA sequences from routinely fixed chromosomal spreads
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چکیده
منابع مشابه
PCR amplification of specific DNA sequences from routinely fixed chromosomal spreads.
The growing field of application of the PCR prompted us to adapt this methodology as a routine screening for cytogenetic preparations. Cytogenetic laboratories usually store fixed cell pellets in fixative (methanol/glacial acetic acid: 3/1) a t 20°C. DNA from these fixed cell pellets can be extracted and analysed (1). However, for a large number of samples, fixed metaphase spreads on glass slid...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.8.1946